Speedily get sections as slender or thick as your software demands, with no stressing about getting rid of or distorting beneficial tissue samples.
Megatome is usually a vibrating microtome designed to segment a broad variety of samples, from organoids and biopsy samples to expanded rodent brains and intact human organs. With significant blade vibrating frequency and minimized blade deflection, Megatome permits superior-throughput tissue sectioning with uniform surface profile, as well as minimum tissue injury and data reduction.
Antibodies could get weeks to diffuse by means of just a few millimeters of tissue, using a steep labeling gradient from surface area to core.
Functioning the CLI straight from a Gradle endeavor will not be at this time supported. A distribution must be made by means of gradlew :j2d-cli:distZip to produce a zip file made up of all the things required to operate.
SE makes use of a rotational electrical area to disperse extremely electromobile molecules (such as antibodies or surfactant micelles) all over a porous sample without the need of harming electrically billed constructions inside the tissue. This enables two-four day clearing of intact organs,
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Megatome can be a novel microtome that permits for top-precision sectioning of an array of tissue samples – from organoids, to arrays of animal organs, to intact human brain hemispheres – with small tissue harm and knowledge reduction.
eFLASH is a swift tissue labeling method which allows for uniform complete-organ staining in twenty rounds of labeling.
The SmartSPIM light sheet microscope delivers unparalleled resolution and acquisition pace all over intact tissue volumes, although megatomi.com its modular structure lets you upgrade and personalize individual parts.
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SHIELD avoids the variability of hydrogel embedding and the knowledge decline from PFA preservation, guarding specimens for a number of rounds of processing.
Leverage the Crystal clear+ tissue clearing method, together with eFLASH and patented stochastic electrotransport technologies, to speedily apparent and label whole organs. Important highlights and functions include things like:
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